ab Assignment: Nuclear Magnetic Resonance

1. Inspect the equipment to check if indeed all of the blue coaxial cables are connected properly. Most likely you will not need to adjust any of the cables but be aware that someone may have inadvertently left something connected improperly. Knowing where all the wires go will also help you familiarize yourself with the equipment.
2. Use the NMR probe to check that the spectrometer is properly calibrated. This part is described in the "Getting Started" section. PLEASE DO NOT adjust the capacitors until you have obtained the signal shown on page II-19 of the manual and you have consulted with the instructor. This calibration step will be discussed during the orientation presented by the instructor. Also, it is relatively easy to break the fragile capacitors which will render the equipment useless. Note the voltage readings during the orientation and make sure they are within expectations.
3. Replace the probe with the sample of mineral oil and attempt to find the FID signal on the scope. A nice signal is illustrated on page III-7 of the PS-2 manual. Record the voltage and time sweep on the oscilloscope. Do you understand what these mean? Are they as expected for the sample at hand?
4. During the search for the FID signal play with the spectrometer settings and look through the questions on page III-7. Try to answers them as you progress through the experiment. At first, they may not make sense but by the end of the experiment, most of them should be clear. Do not worry about answering them on your report. But if you would like to comment please feel free to do so.
5. During the experiment, if you feel compelled to include a particular scope display, say the FID, spin-echo or anything else you may do so in one of two ways: take a photo of the scope screen with your phone, or use the USB port to dump the contents of the scope via the out interface. See the scope manuals on the table in the lab.

• PS2_Conceptual_Introduction_to_the_Experiment.pdf (Links to an external site.): Nice summary of the physics and measurement of T1 and T2 with the PS2-A.
• PS2_Manual_1.41.pdf (Links to an external site.): Revised Teachspin PS2-A manual. It supersedes the old manual and a great improvement.
• Pulsed_NMR.pdf (Links to an external site.): Document that features the PS1A experiment, a precursor to the one you will be using, but with an excellent and clear description of the physics, the experimental setup and measurement of two relaxation times T1 and T2. A good supplement to the new manual
• PS2_Aliasing_on_the_Digital_Oscilloscope_Rev_2.0.pdf (Links to an external site.): A note on scope usage that may be a worthwhile read.

Turning in your lab report

Since this lab is on the rather complicated topic of Nuclear Magnetic Resonance a thorough job of describing the physics behind NMR is expected in the introduction along with a detailed explanation of how one extracts the T1 and T2 relaxation times from measurement on the FID and the spin-echo signal. How were the pulse sequences used to extract these values.

Upload your lab report (article) to the Turnitin via the gray box under the "This tool needs to be loaded in a new browser window" line below. It will be graded along the rubric described in this page Grading Rubric. Read through the rubric carefully to maximize your score. If you have any questions please ask. Remember lab reports are usually due on Friday evening after you complete the experiment in class. If you have questions please ask.

Turning in your coding project

Part B. Find the relaxation times T1 and T2 in a sample of mineral oil

1. Inspect the equipment to check if indeed all of the blue coaxial cable are connected and connected properly. Most likely you won't have to do anything here but be aware that someone may have inadvertently left something connected improperly. Knowing where all the wires go will also help you familiarize yourself with the equipment.
2. Use the NMR probe to check that the spectrometer is properly calibrated. This part is described in the "Getting Started" section. PLEASE DO NOT adjust the capacitors until you've obtained the signal shown on page II-19 of the manual and you have consulted with the instructor. This calibration step will be discussed during the orientation presented by the instructor. Also, it is relatively easy to break the fragile capacitors which will render the equipment useless. Note the voltage readings during the orientation and make sure they are within expectations.
3. Replace the probe with the sample of mineral oil and attempt to find the FID signal on the scope. A nice signal is illustrated on page III-7 of the PS-2 manual. Record the voltage and time sweep on the oscilloscope. Do you understand what these mean? Are they as expected for the sample at hand?
4. During the search for the FID signal play with the spectrometer settings and look through the questions on page III-7. Try to answers them as you progress through the experiment. At first they may not make sense but by the end of the experiment most of them should be clear. Don't worry about answering them on your report. But if you'd like to comment on them on the report please feel free to do so.
5. During the experiment, if you feel compelled to include a particular scope display, say the FID, spin echo or anything else that catches your eye you can do so in one of two ways: the simplest way is to just a take a photo of the scope screen with your phone. Or you can, with our new Tektronix scope also has a USB port you can use to record the output of the scope, in a supported image format.

• Youtube video on Magnetic Resonance using earth-field NMR experiment. The technology employed is different but the discussion is, particularly the theoretical description is appropriate to the Teachspin device http://www.youtube.com/user/magritek

• A nice simulation from the UF's Center for Safety, Simulation and Advanced Learning Technologies. It provides you with a better visual of how NMR works. You'll need an Adobe Shockwave so you iPad/iPhone may not work and or you'll need to add it to you browser. http://vam.anest.ufl.edu/simulations/nuclearmagneticresonance.php

• PS2_Manual_1.41.pdf: Revised Teachspin PS2-A manual. It supersedes the old manual and a great improvement.
• Pulsed_NMR.pdf: Document that features the PS1A experiment, a precursor to the one you will be using, but with an excellent and clear description of the physics, the experimental setup and measurement of two relaxation times T1 and T2. A good supplement to the new manual

1. Now attempt to find the FID signal on page III-7 on the oscilloscope. Use the prepared mineral oil provided. Record the voltage and time sweep on the oscilloscope. Do you understand what these mean? Are they as expected for the sample at hand?
2. During the search for the FID signal play with the spectrometer settings and look through the questions on page III-7. Try to answers them as you progress through the experiment. At first they may not make sense but by the end of the experiment most of them should be clear. Don't worry about answering them on your report, unless you feel compeled to do so.
3. Make sure you record the oscilloscope output. The easiest way to do this is to take a photo of the scope screen with your phone. The new Tektronix scope also has a USB port you can use to record the output of the scope, in a supported image format.

• PS2_Conceptual_Introduction_to_the_Experiment.pdf: Nice summary of the physics and measurement of T1 and T2 with the PS2-A.
• PS2_Manual_1.41.pdf: Revised Teachspin PS2-A manual. It superseeds the old manual and a great improvement.
• Pulsed_NMR.pdf: Document that features the PS1A experiment, a precurser to the one you will be using, but with an excellent and clear description of the physics, the experimental setup and measurment of two relaxation times T1 and T2. A good supplement to the new manual
• PS2_Aliasing_on_the_Digital_Oscilloscope_Rev_2.0.pdf: A note on scope usage that may be a worthwhile read.

Part A. Find the Free Induction Decay in mineral oil

Part B. Find the relaxation times T1 and T2 in your samples of mineral oil

The PS_NMR.pdf file linked below has a nice description on how to perform this measurement. Ask if you have questions.

1. Use the NMR probe to check that the spectrometer is properly calibrated. This part is described in the "Getting Started" section. PLEASE DO NOT adjust the capacitors until you've obtained the signal shown on page II-19 of the manual and you have consulted with me. If this calibration is not carried out properly it will be difficult to find FID signal and it is easy to do this incorrectly. Record the voltage and time sweep observed. Do they correspond with what you expect?
2. Now attempt to find the FID signal on page III-7 on the oscilloscope. Use the prepared mineral oil and water samples provided. Record the voltage and time sweep on the oscilloscope. Do you understand what these mean? Are they as expected for the sample at hand?

1. Make sure you record the oscilloscope output. The easiest way to do this is to take a photo of the scope screen with your phone.
2. We will attempt to measure relaxation times this semester for the first time. I will help you do this.

• Pulsed_NMR.pdf: Document that features the PS1A experiment, a precurser to the one you will be using, but with an excellent and clear description of the physics and experimental setup and the meaning of the two relaxation times T1 and T2. A MUST READ...

Laboratory procedure:

1. Use the NMR probe to check that the spectrometer is properly calibrated. This part is described in the "Getting Started" section. PLEASE DO NOT adjust the capacitors before until you've obtained the signal shown on page II-19 and you have consulted me. If the calibration si not properly done you will never get the FID signal and it is easy to do this incorrectly. Record the voltage and time sweep observed. Do they correspond with what you expect?
2. Now attempt to find the FID signal on page III-7 on the oscilloscope. Us the prepared mineral oil and water samples provided. Record the voltage and time sweep on the oscilloscope. Do you understand what these mean? Are they as expected for the sample at hand?
3. During the search for the FID signal play with the spectrometer settings and answer the questions highlighted in yellow on page III-7.
4. Make sure you record the oscilloscope output. Our oscilloscope has a flopy drive and a network jack as output channels. You are more than welcomed to use either of these to output the display. However, its probably easier and faster to just take a photo of the screen with your phone.

• PS2Lab-manual.pdf: Techspin PS2-A NMR manual

Lab Assignment: Nuclear Magnetic Resonance

In this lab you will explore nuclear magnetic resonance with the Teachsping Pulsed/CW NMR Spectrometer.

Part A. Find the Free Induction Decay in mineral oil and water

Before you begin you will need to familiarize yourself with the equipment by carefully reading the manual provided below. You are encouraged to read the theory and experimental apparatus description thourougly before you turn on the spectrometer. It may also be beneficial to read through and understand qualatatively how an oscilloscope works. For this lab you will be using the smaller of the two oscilloscopes in the locked cabinet, the Tektronix TDS 2002B. Its not as sophisticated as the oscilloscope you used for the CountingLab but it should suffice for this experiment.

The manual for the spectrometer is available PulsedNMRTechSpinManual.pdf: Teachspin PS2-A NMR manual

Laboratory proceedure:

1. Read the manual attached below before you start
2. Connect all coaxial cable not already connected. The manual describes how to do this. Most likely you'll just need to do connect the spectrometer to the oscilloscope.
3. Turn on and tune the permanent magnetic temperature control system, see page section II.12 - II.15
4. Use the NMR probe to calibrate the spectrometer as described in the "Getting Started" section
5. Now attempt to find the FID signals in the prepared mineral oil and water samples provided

• You Tube video on Magnetic Resonance using earth-field NMR experiment. The technology employed is different but the discussion is, particularly the theoretical description is appropriate to the Teachspin device http://www.youtube.com/user/magritek
• This online book by Joseph P. Hornak, Ph.D. is something worthwhile to peruse http://www.cis.rit.edu/htbooks/nmr/inside.htm
• A nice simulation from the UF's Center for Safety, Simulation and Advanced Learning Technologies. It provides you with a better visual of how NMR works. You'll need an Adobe Shockwave so you ipad/iphone may not work and or you'll need to add it to you browser. It worked from me from explorer but not with chrome. http://vam.anest.ufl.edu/simulations/nuclearmagneticresonance.php